Fig. 2.

Spontaneous GABAergic post-synaptic currents in the NCM are not affected by song exposure. a Representative sIPSCs recorded at −70 mV using a high-chloride internal solution in the presence of DNQX. b i sIPSC frequency in the NCM of control and song-exposed birds (control: 3.2 ± 0.4 Hz, n = 23; song-exposed: 3.4 ± 0.4 Hz, n = 20), b ii sIPSC frequency in the NCM of control and song-exposed birds segregated by gender (control females: 3.4 ± 0.5 Hz, n = 11; song-exposed females: 2.8 ± 0.7 Hz, n = 8; control males: 3.1 ± 0.6 Hz, n = 12; song-exposed males: 3.9 ± 0.6 Hz, n = 12). c i sIPSC amplitude in the NCM of control and song-exposed birds (control: 41.1 ± 3.6 pA, n = 23; song-exposed: 37.5 ± 2.6 pA, n = 20). c ii sIPSC amplitude in the NCM of control and song-exposed birds segregated by gender (control females: 40.6 ± 5 pA, n = 11; song-exposed females: 37.9 ± 4 pA, n = 8; control males: 41.7 ± 5 pA, n = 12; song-exposed males: 37.2 ± 3 pA, n = 12). d Representative sIPSCs recorded at −20 mV using a low-chloride internal solution. Outward currents are indicated by asterisks and inward currents by arrowheads. e sIPSC frequency measured at −20 mV with a low-chloride internal solution (control: 2.3 ± 0.5 Hz, n = 9, 6 males and 3 females; song-exposed: 2.0 ± 0.4 Hz, n = 12, 6 males and 6 females). f sIPSC amplitude measured at −20 mV in a low-Cl internal solution (control: 25.3 ± 3 pA, n = 9, 6 males and 3 females; song-exposed: 20.9 ± 3 pA, n = 12, 6 males and 6 females). Values above correspond to the mean ± SEM; on the graphs, each symbol corresponds to a value of a single neuron and the horizontal line represents the mean