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Effect of 5-ASA on mononucleotide and tetranucleotide microsatellites. The effect of 5-ASA on replication fidelity within mononucleotide repeat sequences A10, G10 (A), and (AAAG)17 repeats (B) was tested. Nonfluorescent cells (5 × 103) were sorted into six-well plates and treated with 5 mmol/L 5-ASA for a period of 8 d. The EGFP-positive mutant fraction was analyzed by flow cytometry. Columns, mean of triplicate cultures for each clone; bars, SEM.
