Fig. 1.

Effects on mice pathophysiology after treatment with VPA by intraperitoneal injection

N=3 for each group treated with VPA under 100 to 300 mg/kg, twice daily (bid); N=6 for the group under the dosage of 400 mg/kg, bid. (a) Mice liver function detected after 2 d of VPA administration under the dosage of 100 to 400 mg/kg, i.p. bid (^* P<0.05 when compared with normal conditions). (b) Histology of vital organs stained with HE after VPA treatment (300 mg/kg, i.p. bid) for 2 d. (c) Western blot analysis of histone acetylation state detected with antibody acH3 at 0, 12, 24, 36, 48, 72, 120, or 168 h after VPA treatment (300 mg/kg, i.p. bid). The levels of total H3 were also detected at the same time points. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was used as a loading control. The lower panel shows a densitometry histogram of acH3