FIGURE 1.

Effects of L48Q on the Ca²⁺ dependent changes in the fluorescence of $\text{cTn}\frac{C35S}{IANBD}$ complexes. (◯) Ca²⁺binding to L48Q $\text{cTn}\frac{C35S}{IANBD}$; (•) Ca²⁺ binding to $\text{cTn}\frac{C35S}{IANBD}$. Inset graph, effects of L48Q on the total magnitude IANBD fluorescence increase. Excitation was at 490 nm and the emission was monitored at 530 nm. The error bars represent the standard error of 3–5 experiments.p<0.05