Table 3.
Main objectives and achievements of the EuroFlow project
| Objectives | |
|---|---|
| 1. | Development of novel antibodies, particularly against intracellular proteins, such as oncoproteins and newly defined classification markers, as identified by gene expression profiling and molecular cytogenetic findings |
| 2. | Novel immunobead technology for fast and easy classification of acute leukemias via detection of oncogenic fusion proteins in cell lysates |
| 3. | Novel flow cytometry software for easy and fast handling and integration of list mode data files of multi-tube 8-color immunostainings and for automated pattern recognition of novel, reactive/regenerating and malignant cell populations |
| 4. | Evaluation and selection of fluorochromes suited for 8-color flow cytometric immunostainings |
| 5. | Development of standardized procedures for instrument settings and immunostaining procedures to guarantee reliable interlaboratory comparability of flow cytometric immunophenotyping |
| 6. | Design, standardization and clinical evaluation of novel 8-color immunostaining protocols for diagnosis and classification of hematopoietic malignancies |
| 7. | Design, standardization and clinical evaluation of 8-color immunostaining protocols for assessment of treatment effectiveness via monitoring the malignant cells during and after treatment |
| Achievements/deliverables | |
| 1. | Development of new antibodies directed to oncogene proteins and tumor-associated markers |
| 2. | Novel immunobead assays for detection of fusion proteins |
| 3. | Novel software for integration of list mode data files and multivariate data analysis |
| 4. | Standardized procedures for instrument set-up and 8-color flow cytometry immunostaining optimized for immunophenotyping of leukemias and lymphomas |
| 5. | New evaluated EuroFlow antibody panels for diagnosis and classification of leukemias and lymphomas |
| 6. | Educational program (EuroFlow workshopsa, web pageb and scientific publications) |
aPlease see Table 4.