Table 3. Mean fluorescence intensity (MFI) and stain index (SI) values obtained for different sets of reagents evaluated in normal PB samples (n=5).

Marker		PacB	HV450	P-valuea
CD2	Clone (manufacturer)	TS1/8 (BioLegend)	S5.2 (BD B)
	MFI±s.d. of CD2+ T and NK-cells	5741±755.7	8259±1792.8	0.02
	SIc	37.0	41.4
CD3	Clone (manufacturer)	UCHT1 (BD Ph)	UCHT1 (BD B)
	MFI±s.d. of T-cells	15 774±1503.7	17 246±814.2	0.08	APCCy7	AF700	APCH7	P-valueb
	SId	117.2	130.5
CD4	Clone (manufacturer)	RPA-T4 (BD Ph)	RPA-T4 (BD B)		RPA-T4 (BD B)	RPA-T4 (BD B)	RPA-T4 (BD B)
	MFI±s.d. of CD4+ T-cells	9474±710.2	9195±408.2	0.46	13 596±686.5	4307±174.1	9910±414.3	<0.001
	SIe	61.8	66.6		42.6	41.4	35.0
CD20	Clone (manufacturer)	2H7 (eBiosciences)	L27 (BD B)
	MFI±s.d. of B-cells	30 073±3783.5	38 152±2857.4	0.005	PacO	AmCyan	HV500	P-valueb
	SIf	219.61	222.8
CD45	Clone (manufacturer)	T29/33 (Dako)	HI30 (BD B)		HI30 (Invitrogen)	2D1 (BD B)	HI30 (BD B)
	MFI±s.d. of lymphocytes	30 742±824.8	53 709±2062.2	<0.001	5521±150.6	30 681±2838.3	19 157±686.3	<0.001
	SIg	3.9	5.2		3.8	4.8	3.8
HLADR	Clone (manufacturer)	L243 (BioLegend)	L243 (BD B)
	MFI±s.d. of monocytes	11 509±1721.4	16 874±1934.3	0.002
	SIh	47.7	64.8

Abbreviations: AF700, alexa fluor 700; AmCyan, Anemonia Majano cyan fluorescent protein; APCCy7, allophycocyanin–cyanin7; APCH7, allophycocyanin–hilite7; BD Ph, BD Pharmingen; BD B, BD Biosciences; HV450, Horizon V450; HV500, Horizon V500; PacB, pacific blue; PacO, pacific orange.

^aPaired Student's T-test.

^bP<0.001 for the following comparisons: APCH7 versus AF700, AF700 versus APCCy7, APCCy7 versus APCH7, PacO versus AmCyan, PacO versus HV500 and AmCyan versus HV500 (paired Student's T-test).

^cPositive reference population (PRP): CD2⁺ T- and NK-cells and negative reference population (NRP), CD2⁻ lymphocytes.

^dPRP, T-cells; NRP, B- and NK-cells.

^ePRP, CD4⁺ T-cells; NRP, CD4⁻ T-cells.

^fPRP, B-cells; NRP, T- and NK-cells.

^gPRP, lymphocytes; NRP, neutrophils.

^hPRP, monocytes; NRP, lymphocytes.