Table 4. Target mean fluorescence intensity (MFI) values obtained after optimal PMT adjustments for each fluorescence channel for the brightest peak of Rainbow 8-peak calibration beads in the LSR II instrument.
| Fluorochrome channel |
MFI values Rainbow lot no. |
||
|---|---|---|---|
| X02, Y02 | Z02 | EAB01 | |
| PacB | 195 572 | 194 818 | 215 352 |
| PacO | 231 265 | 216 293 | 217 908 |
| FITC | 59 574 | 58 372 | 65 283 |
| PE | 101 900 | 98 520 | 84 847 |
| PerCPCy5.5 | 216 064 | 223 940 | 228 818 |
| PECy7 | 27 462 | 27 185 | 29 865 |
| APC | 176 780 | 226 435 | 252 000 |
| APCH7 | 56 437 | 81 371 | 102 099 |
Abbreviations: APC, allophycocyanin; Cy7, cyanin7; FITC, fluorescein isothiocyanate; H7, hilite7; PacB, pacific blue; PacO, pacific orange; PE, phycoerythrin; PerCPCy5.5, peridinin–chlorophyll–protein–cyanin5.5.