Table 14. The EuroFlow AML/MDS antibody panela b.
| Tube | PacB | PacO | FITC | PE | PerCPCy5.5 | PECy7 | APC | APCH7 | Aim |
|---|---|---|---|---|---|---|---|---|---|
| 1 | HLADR | CD45 | CD16 | CD13 | CD34 | CD117 | CD11b | CD10 | Diagnosis and classification, neutrophilic maturation, PNH |
| 2 | HLADR | CD45 | CD35 | CD64 | CD34 | CD117 | CD300e (IREM2) | CD14 | Diagnosis and classification, monocytic maturation, PNH |
| 3 | HLADR | CD45 | CD36 | CD105 | CD34 | CD117 | CD33 | CD71 | Diagnosis and classification, erythroid maturation |
| 4 | HLADR | CD45 | NuTdT | CD56 | CD34 | CD117 | CD7 | CD19 | Aberrant expression of lymphoid markers, abnormal B lymphoid maturation |
| 5 | HLADR | CD45 | CD15 | NG2 | CD34 | CD117 | CD22 | CD38 | Aberrant marker expression, stem cells |
| 6 | HLADR | CD45 | CD42a and CD61 | CD203c | CD34 | CD117 | CD123 | CD4 | Diagnosis and classification of AML Megakaryocytic, basophilic, and plasmacytoid dendritic cell lineages |
| 7 | HLADR | CD45 | CD41 | CD25 | CD34 | CD117 | CD42b | CD9 | Characterization of megakaryoblastic leukemia, and systemic mastocytosis |
Abbreviations: AML, acute myeloid leukemia; APC, allophycocyanin; BB, backbone; BM, bone marrow; Cy7, cyanin7; FITC, fluorescein isothiocyanate; H7, hilite7; MDS, myelodysplastic syndrome; Nu, nuclear; PacB, pacific blue; PacO, pacific orange; PE, phycoerythrin; PerCPCy5.5, peridinin–chlorophyll–protein–cyanin5.5; PNH, paroxysmal nocturnal hemoglobinuria.
a
Further information about markers and hybridomas is provided in the Appendix.
b
A total of 96 BM samples were evaluated for selection of the BB markers. An additional 84 BM AML samples were evaluated with this version (final) of the panel.