Table A1. Composition of ALOT and technical information on reagents.
|
PacB |
PacO |
FITC |
PE |
PerCPCy5.5 |
PECy7 |
APC |
APCH7 |
|
| CyCD3 | CD45 | CyMPO | CyCD79a | CD34 | CD19 | CD7 | SmCD3 |
| Marker | Fluorochrome | Clone | Source | Catalogue number | (μl/test) |
|---|---|---|---|---|---|
| CyCD3 | PacB | UCHT1 | BD Biosciences | 558117 | 7 |
| SmCD3 | APCH7 | SK7 | BD Biosciences | 641397 | 3 |
| CD7 | APC | 124-1D1 | eBioscience | 17-0079-42 | 2 |
| CD19 | PECy7 | J3-119 | Beckman Coulter | IM3628 | 5 |
| CD34 | PerCPCy5.5 | 8G12 | BD Biosciences | 347222 | 7 |
| CD45 | PacO | HI30 | Invitrogen | MHCD4530 | 5 |
| CyCD79a | PE | HM57 | Dako | R7159 | 5 |
| CyMPO | FITC | MPO-7 | Dako | F0714 | 3 |