Table A10. Composition of NK-CLPD panel and technical information on reagents.
|
Tube |
PacB |
PacO |
FITC |
PE |
PerCPCy5.5 |
PECy7 |
APC |
APCH7 |
| 1 | CD2 | CD45 | CD7 | CD26 | SmCD3 | CD56 | CD5 | CD19 |
| 2 | CD16 | CD45 | CD57 | CD25 | SmCD3 | CD56 | CD11c | CD19 |
| 3 | HLADR | CD45 | CyPerforin | CyGranzyme B | SmCD3 | CD56 | CD94 | CD19 |
| Marker | Fluorochrome | Clone | Source | Catalogue number | (μl/test) |
|---|---|---|---|---|---|
| CD2 | PacB | TS1/8 | Biolegend | 309216 | 1 |
| SmCD3 | PerCPCy5.5 | SK7 | BD Biosciences | 332771 | 10 |
| CD5 | APC | L17F12 | BD Biosciences | 345783 | 2.5 |
| CD7 | FITC | 4H9 | BD Biosciences | 347483 | 10 |
| CD11c | APC | S-HCL-3 | BD Biosciences | 333144 | 2 |
| CD16 | PacB | 3G8 | Biolegend | 302032 | 5 |
| CD19 | APCH7 | SJ25C1 | BD Biosciences | 641395 | 5 |
| CD25 | PE | 2A3 | BD Biosciences | 341011 | 10 |
| CD26 | PE | L272 | BD Biosciences | 340423 | 10 |
| CD45 | PacO | HI30 | Invitrogen | MHCD4530 | 5 |
| CD56 | PECy7 | N901 | Beckman Coulter | A21692 | 5 |
| CD57 | FITC | HNK-1 | BD Biosciences | 333169 | 10 |
| CD94 | APC | HP-3D9 | BD Biosciences | 559876 | 5 |
| CyGranzyme B | PE | CLB-GB11 | Sanquin | M2289 | 15 |
| CyPerforin | FITC | δG9 | BD Biosciences | 556577 | 10 |
| HLADR | PacB | L243 | Biolegend | 307624 | 1 (1:5 dilution) |