Table A2. Composition of LST and technical information on reagents.
|
PacB |
PacO |
FITC |
PE |
PerCPCy5.5 |
PECy7 |
APC |
APCH7 |
|
| CD20 and CD4 | CD45 | CD8 and SmIgλ | CD56 and SmIgκ | CD5 | CD19 and TCRγδ | SmCD3 | CD38 |
| Marker | Fluorochrome | Clone | Source | Catalogue number | (μl/test) |
|---|---|---|---|---|---|
| SmCD3 | APC | SK7 | BD Biosciences | 345767 | 2.5 |
| CD4 | PacB | RPA-T4 | BioLegend | 300521 | 0.5 |
| CD5 | PerCPCy5.5 | L17F12 | BD Biosciences | 341109 | 15 |
| CD8 | FITC | UCH-T4 | Cytognos | CYT-SLPC-50 | Part of LST mixture (20) |
| CD19 | PECy7 | J3-119 | Beckman Coulter | IM3628 | 5 |
| CD20 | PacB | 2H7 | BioLegend | 302320 | 1 |
| CD38 | APCH7 | HB7 | BD Biosciences | 646786 | 3 |
| CD45 | PacO | HI30 | Invitrogen | MHCD4530 | 5 |
| CD56 | PE | C5.9 | Cytognos | CYT-SLPC-50 | Part of LST mixture (20) |
| SmIgκ | PE | polyclonal | Cytognos | CYT-SLPC-50 | Part of LST mixture (20) |
| SmIgλ | FITC | polyclonal | Cytognos | CYT-SLPC-50 | Part of LST mixture (20) |
| TCRγδ | PECy7 | 11F2 | BD Biosciences | 649806 | 1 |