Table A6. Composition of T-ALL panel and technical information on reagents.
|
Tube |
PacB |
PacO |
FITC |
PE |
PerCPCy5.5 |
PECy7 |
APC |
APCH7 |
| 1 | CyCD3 | CD45 | NuTdT | CD99 | CD5 | CD10 | CD1a | SmCD3 |
| 2 | CyCD3 | CD45 | CD2 | CD117 | CD4 | CD8 | CD7 | SmCD3 |
| 3 | CyCD3 | CD45 | TCRγδ | TCRαβ | CD33 | CD56 | CyTCRβ | SmCD3 |
| 4 | CyCD3 | CD45 | CD44 | CD13 | HLADR | CD45RA | CD123 | SmCD3 |
| Marker | Fluorochrome | Clone | Source | Catalogue number | (μl/test) |
|---|---|---|---|---|---|
| CD1a | APC | HI149 | BD Biosciences | 559775 | 5 |
| CD2 | FITC | RPA-2.10 | BD Biosciences | 555326 | 5 |
| CyCD3 | PacB | UCHT1 | BD Biosciences | 558117 | 7 |
| SmCD3 | APCH7 | SK7 | BD Biosciences | 641397 | 3 |
| CD4 | PerCPCy5.5 | SK3 | BD Biosciences | 332772 | 7 |
| CD5 | PerCPCy5.5 | L17F12 | BD Biosciences | 341109 | 15 |
| CD7 | APC | 124-1D1 | eBioscience | 17-0079-42 | 2 |
| CD8 | PECy7 | SFCI21Thy2D3 | Beckman Coulter | 737661 | 5 |
| CD10 | PECy7 | HI10A | BD Biosciences | 341112 | 5 |
| CD13 | PE | L138 | BD Biosciences | 347406 | 7 |
| CD33 | PerCPCy5.5 | P67.6 | BD Biosciences | 333146 | 10 |
| CD44 | FITC | L178 | BD Biosciences | 347943 | 7 |
| CD45 | PacO | HI30 | Invitrogen | MHCD4530 | 5 |
| CD45RA | PECy7 | L48 | BD Biosciences | 337186 | 5 |
| CD56 | PECy7 | N901 | Beckman Coulter | A21692 | 5 |
| CD99 | PE | Tü12 | BD Biosciences | 555689 | 5 |
| CD117 | PE | 104D2 | BD Biosciences | 332785 | 5 |
| CD123 | APC | AC145 | Miltenyi Biotec | 130-090-901 | 7 |
| HLADR | PerCPCy5.5 | L243 | BD Biosciences | 552764 | 10 |
| TCRαβ | PE | IP26A | Beckman Coulter | A39499 | 7 |
| CyTCRβ | APC | 8A3 (βF1) | Cytognos | CYT-BF1AP | 3 |
| TCRγδ | FITC | IMMU510 | Beckman Coulter | IM1571U | 10 |
| NuTdT | FITC | HT-6 | Dako | F7139 | 10 |