Table A7. Composition of AML/MDS panel and technical information on reagents.
| Tube | PacB | PacO | FITC | PE | PerCPCy5.5 | PECy7 | APC | APCH7 |
|---|---|---|---|---|---|---|---|---|
| 1 | HLADR | CD45 | CD16 | CD13 | CD34 | CD117 | CD11b | CD10 |
| 2 | HLADR | CD45 | CD35 | CD64 | CD34 | CD117 | CD300e | CD14 |
| 3 | HLADR | CD45 | CD36 | CD105 | CD34 | CD117 | CD33 | CD71 |
| 4 | HLADR | CD45 | NuTdT | CD56 | CD34 | CD117 | CD7 | CD19 |
| 5 | HLADR | CD45 | CD15 | NG2 | CD34 | CD117 | CD22 | CD38 |
| 6 | HLADR | CD45 | CD42a and CD61 | CD203c | CD34 | CD117 | CD123 | CD4 |
| 7 | HLADR | CD45 | CD41 | CD25 | CD34 | CD117 | CD42b | CD9 |
| Marker | Fluorochrome | Clone | Source | Catalogue number | (μl/test) |
|---|---|---|---|---|---|
| CD4 | APCH7 | SK3 | BD Biosciences | 641398 | 5 |
| CD7 | APC | 124-1D1 | eBioscience | 17-0079-42 | 2 |
| CD9 | APCH7 | M-L13 | BD Biosciences | 646782 | 5 |
| CD10 | APCH7 | HI10A | BD Biosciences | 646783 | 5 |
| CD11b | APC | D12 | BD Biosciences | 333143 | 5 |
| CD13 | PE | L138 | BD Biosciences | 347406 | 7 |
| CD14 | APCH7 | MϕP9 | BD Biosciences | 641394 | 5 |
| CD15 | FITC | MMA | BD Biosciences | 332778 | 10 |
| CD16 | FITC | CLB FcR gran/1, 5D2 | Sanquin | M1604 | 20 |
| CD19 | APCH7 | SJ25C1 | BD Biosciences | 641395 | 5 |
| CD22 | APC | S-HCL-1 | BD Biosciences | 333145 | 5 |
| CD25 | PE | 2A3 | BD Biosciences | 341011 | 10 |
| CD33 | APC | P67.6 | BD Biosciences | 345800 | 10 |
| CD34 | PerCPCy5.5 | 8G12 | BD Biosciences | 347222 | 5 |
| CD35 | FITC | E11 | BD Biosciences | 555452 | 5 |
| CD36 | FITC | CLB-IVC7 | Sanquin | M1613 | 5 |
| CD38 | APCH7 | HB7 | BD Biosciences | 646786 | 3 |
| CD41 | FITC | CLB-tromb/7, 6C9 | Sanquin | M1674 | 1 |
| CD42a | FITC | GRP-P | Serotec | MCA1227F | 1 |
| CD42b | APC | HIP1 | BD Biosciences | 551061 | 1 |
| CD45 | PacO | HI30 | Invitrogen | MHCD4530 | 5 |
| CD56 | PE | C5.9 | Cytognos | CYT-56PE | 5 |
| CD61 | FITC | RUU-PL7F12 | BD Biosciences | 347407 | 4 |
| CD64 | PE | 10.1 | Serotec | MCA756PE | 10 |
| CD71 | APCH7 | M-A712 | BD Biosciences | 646789 | 5 |
| CD105 | PE | 1G2 | Beckman Coulter | A07414 | 10 |
| CD117 | PECy7 | 104D2D1 | Beckman Coulter | IM3698 | 5 |
| CD123 | APC | AC145 | Miltenyi Biotec | 130-090-901 | 10 |
| CD203c | PE | 97A6 | Beckman Coulter | IM3575 | 10 |
| CD300e | APC | UP-H2 | Immunostep | IREM2A-T100 | 5 |
| HLADR | PacB | L243 | Biolegend | 307624 | 1 (1:5 dilution) |
| NG2 | PE | 7.1 | Beckman Coulter | IM3454U | 10 |
| NuTdT | FITC | HT-6 | Dako | F7139 | 10 |