Table A8. Composition of B-CLPD panel and technical information on reagents.
Tube |
PacB |
PacO |
FITC |
PE |
PerCPCy5.5 |
PECy7 |
APC |
APCH7 |
1 | CD20 and CD4 | CD45 | CD8 and Igλ | CD56 and Igκ | CD5 | CD19 and TCRγδ | SmCD3 | CD38 |
2 | CD20 | CD45 | CD23 | CD10 | CD79b | CD19 | CD200 | CD43 |
3 | CD20 | CD45 | CD31 | CD305 | CD11c | CD19 | SmIgM | CD81 |
4 | CD20 | CD45 | CD103 | CD95 | CD22 | CD19 | CD185 | CD49d |
5 | CD20 | CD45 | CD62L | CD39 | HLADR | CD19 | CD27 |
Marker | Fluorochrome | Clone | Source | Catalogue number | (μl/test) |
---|---|---|---|---|---|
SmCD3 | APC | SK7 | BD Biosciences | 345767 | 2.5 |
CD4 | PacB | RPA-T4 | BioLegend | 300521 | 0.5 |
CD5 | PerCPCy5.5 | L17F12 | BD Biosciences | 341109 | 15 |
CD8 | FITC | UCH-T4 | Cytognos | CYT-SLPC-50 | Part of LST mixture (20) |
CD10 | PE | ALB1 | Beckman Coulter | A07760 | 20 |
CD11c | PerCPCy5.5 | B-Ly6 | BD Biosciences | 646784 | 5 |
CD19 | PECy7 | J3-119 | Beckman Coulter | IM3628 | 5 |
CD20 | PacB | 2H7 | BioLegend | 302320 | 1 |
CD22 | PerCPCy5.5 | S-HCL-1 | BD Biosciences | 649804 | 2 |
CD23 | FITC | MHM6 | Dako | F7062 | 2.5 |
CD27 | APC | L128 | BD Biosciences | 337169 | 2.5 |
CD31 | FITC | WM59 | BD Biosciences | 555445 | 10 |
CD38 | APCH7 | HB7 | BD Biosciences | 646786 | 3 |
CD39 | PE | TÜ66 | BD Biosciences | 555464 | 10 |
CD43 | APCH7 | IG10 | BD Biosciences | 646787 | 5 |
CD45 | PacO | HI30 | Invitrogen | MHCD4530 | 5 |
CD49d | APCH7 | 9F10 | BD Biosciences | 646788 | 2 |
CD56 | PE | C5.9 | Cytognos | CYT-SLPC-50 | Part of LST mixture (20) |
CD62L | FITC | SK11 | BD Biosciences | 347443 | 2.5 |
CD79b | PerCPCy5.5 | SN8 | BD Biosciences | 646790 | 5 |
CD81 | APCH7 | JS-81 | BD Biosciences | 646791 | 5 |
CD95 | PE | DX2 | BD Biosciences | 555674 | 20 |
CD103 | FITC | Ber-ACT8 | BD Biosciences | 333155 | 2 |
CD185 | APC | 51505 | R&D Systems | FAB190A | 10 |
CD200 | APC | OX104 | eBioscience | 17-9200 | 1.25 |
CD305 | PE | DX26 | BD Biosciences | 550811 | 10 |
HLADR | PerCPCy5.5 | L243 | BD Biosciences | 552764 | 10 |
SmIgκ | PE | Polyclonal | Cytognos | CYT-SLPC-50 | Part of LST mixture (20) |
SmIgλ | FITC | Polyclonal | Cytognos | CYT-SLPC-50 | Part of LST mixture (20) |
SmIgM | APC | G20-127 | BD Biosciences | 551062 | 10 |
TCRγδ | PECy7 | 11F2 | BD Biosciences | 649806 | 1 |