Table 1.
CHD4 SAXS parameters
| Construct | Concentration (mg/mL) | RgautoRg (nm) | RgGNOM (nm) | Dmax (nm) | MMSAXS (kDa) |
|---|---|---|---|---|---|
| PP-CC-AH-D (117 kDa) | 8.3 | 5.6 ± 0.1 | 5.7 ± 0.1 | 19.6 ± 0.5 | 142 ± 15 |
| 4.9 | 5.3 ± 0.1 | 5.4 ± 0.1 | 18.4 ± 0.5 | 128 ± 10 | |
| 2.0 | 5.0 ± 0.1 | 5.2 ± 0.1 | 17.3 ± 0.5 | 121 ± 10 | |
| 1.0 | 5.0 ± 0.1 | 5.0 ± 0.1 | 17.1 ± 0.5 | 121 ± 10 | |
| mer | 4.9 ± 0.1 | 5.0 ± 0.1 | 17.4 ± 0.5 | 119 ± 10 | |
| CC-AH-D (101 kDa) | 7.1 | 4.5 ± 0.1 | 4.6 ± 0.1 | 15.9 ± 0.5 | 93 ± 10 |
| 4.1 | 4.3 ± 0.1 | 4.4 ± 0.1 | 14.9 ± 0.5 | 87 ± 10 | |
| 2.2 | 4.2 ± 0.1 | 4.2 ± 0.1 | 14.5 ± 0.5 | 81 ± 10 | |
| 1.0 | 4.0 ± 0.1 | 4.1 ± 0.1 | 14.2 ± 0.5 | 78 ± 10 | |
| mer | 4.0 ± 0.1 | 4.0 ± 0.1 | 14.5 ± 0.5 | 77 ± 10 | |
| AH (62.9 kDa) | 8.6 | 6.5 ± 0.1 | 6.6 ± 0.1 | 21.9 ± 0.5 | 99 ± 10 |
| 3.8 | 4.8 ± 0.1 | 5.0 ± 0.1 | 16.9 ± 0.5 | 66 ± 10 | |
| 2.1 | 4.4 ± 0.1 | 4.3 ± 0.1 | 14.7 ± 0.5 | 57 ± 10 | |
| 1.4 | 4.3 ± 0.1 | 4.2 ± 0.1 | 14.8 ± 0.5 | 53 ± 10 | |
| mer | 4.2 ± 0.1 | 4.0 ± 0.1 | 14.5 ± 0.5 | 52 ± 10 | |
| PP-CC (37.9 kDa) | 8.2 | 4.0 ± 0.1 | 4.1 ± 0.1 | 13.9 ± 0.5 | 56 ± 10 |
| 4.7 | 3.7 ± 0.1 | 3.9 ± 0.1 | 13.1 ± 0.5 | 49 ± 10 | |
| 2.7 | 3.5 ± 0.1 | 3.6 ± 0.1 | 12.2 ± 0.5 | 37 ± 10 | |
| 3.3 | 3.5 ± 0.1 | 3.6 ± 0.1 | 12.2 ± 0.5 | 46 ± 10 | |
| 1.1 | 3.2 ± 0.1 | 3.3 ± 0.1 | 11.3 ± 0.5 | 40 ± 10 | |
| mer | 3.0 ± 0.1 | 3.1 ± 0.1 | 10.5 ± 0.5 | 39 ± 10 |
RgautoRg and RgGNOM are the radii of gyration estimated from the SAXS data using the automated Guinier analysis routine and the GNOM program, respectively. Dmax and MMSAXS are the maximum particle dimension and molecular mass estimated from I(0). “mer” refers to the merged SAXS curve from all data sets that was extrapolated to infinite dilution.