Table 2.
Kinetic parameters of choline and albitiazolium entry into P. falciparum free parasites and their cross-inhibition
| Choline entry | Albitiazolium entry | |
|---|---|---|
| Km (µM) | 18.5 ± 3.3 | 14.2 ± 2.6 |
| Vmax (pmol per 107 cells·min−1) | 23.3 ± 4.5 | 6.1 ± 1.1 |
| Inhibitors | Albitiazolium | Choline |
|---|---|---|
| IC50 (µM) | 2.3 ± 0.2 | 808 |
| Ki (µM) | 0.53 | 321 |
| Type of inhibition | Competitive | Competitive |
Values are means of at least three independent experiments performed in duplicate (SEM is indicated). Otherwise, values are means of two independent experiments. IC50s were determined using 15 µM choline and 16 µM albitiazolium; concentrations that correspond to their Km.