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. Author manuscript; available in PMC: 2012 Sep 10.
Published in final edited form as: Cell Rep. 2012 Mar 8;1(3):241–250. doi: 10.1016/j.celrep.2012.01.006

Figure 1. Polycystins and mechanical stress-induced PCT cell death.

Figure 1

A) Early apoptosis detected by Annexin V labeling (visualized in green as shown on the right panels) induced by mechanical stress (4 hours at ~2800 g) in both Pkd1lox/− (top) and Pkd1−/− (bottom) cultured plated PCT cells. Total number of nuclei and late cell death are detected by a Hoechst staining (shown in black and white on the left panels). B) Histogram showing the amount of early (Annexin V) and late (Hoechst) apoptosis induced by mechanical stress in both Pkd1lox/− (n = 18 plates with 19 000 cells analyzed) and Pkd1−/− PCT cells (n = 18 plates with 36 000 cells analyzed). C) Histogram showing the amount of late apoptosis (Hoechst staining) induced by mechanical stress in both mock- (n = 12 plates with 1300 transfected cells analyzed) and PC2-740X (n = 11 plates with 250 transfected cells analyzed) transiently transfected cultured PCT cells. Transfected cells were visualized by EGFP fluorescence (green) and nuclear fragmentation evaluated by Hoechst staining. Cell death was determined 3 hours after mechanical stress. Data represent mean ± standard error of the mean.