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. 2012 May 1;20(9):1689–1698. doi: 10.1038/mt.2012.83

Figure 2.

Figure 2

Stability comparisons of T5.0000, T5.0001, T5.0002, and T5.0007 (see Table 1) upon serial passage on human U87 glioblastoma cells. Passage conditions are described in the text. The figure shows the relative stability of the integrated proviruses at 12 serial passages (P1–P12) by displaying at each passage the PCR amplification product across the IRES transgene from DNA from infected cells after gel electrophoresis. The positive control amplified directly from the plasmid is shown on the far left of each panel, and a size marker is in the next lane, with sizes as shown. Bands with mobility greater than that of the positive control indicate the appearance of replicating viruses with deletions in the IRES-transgene region. The sizes of the amplified bands from the original plasmids are T5.0000: 1,040 bp; T5.0002: 1,038 bp; T5.0001: 1,041 bp; T5.0007: 1,039 bp. Corresponding stability tests for T5.0003, T5.0004, T5.0005, and T5.0006 are shown in the Supplementary Materials and Methods. IRES, internal ribosome entity site.