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. 2012 May 15;20(9):1800–1809. doi: 10.1038/mt.2012.98

Figure 4.

Figure 4

DCLV prime/DCLV boost in combination with PD-L1 blockade generated prolonged immunity. (a) Schematic representation of experimental procedures. (b) The percentage of Gag tetramer-positive CD8+ T cells in PBMC was determined at the indicated time points. Day 0 denotes the date that mice (n = 4) were boosted with DCLV-Gag plus αPD-L1 or isotype antibody treatment. At day 68 post-boost immunization, splenocytes were harvested from different groups of mice for analysis. (c) Representative FACS plots for measuring the population of tetramer-positive CD8+ T cells. (d) The percentages of cytokine-secreting CD8+ T cells upon restimulation with HIV-1 Gag peptide pools are shown. (e) Cytokine coexpression subsets are expressed as a percentage of total CD8+ T cells. P values were calculated using one-way Anova followed by a Bonferroni nonparametric posttest (ns, not significant; *P < 0.05; **P < 0.01; ***P < 0.001). The data are representative of two independent experiments. DCLV, dendritic cell-directed lentiviral vector; HIV, human immunodeficiency virus; PBMC, peripheral blood mononuclear cell; PBS, phosphate-buffered saline; PD-L1, programmed death 1 ligand, SFC, spot-forming cells.