Figure 5. The H8 Loop of Kapβ2 Is Mostly Disordered.

The 2F_o – F_c map (stereo diagram, 1.0σ, blue mesh) of unliganded Kapβ2 at the H8 loop (yellow) and C-terminal arch (blue) shows that most of the loop is disordered and not bound in the C-terminal arch. H8 loop (yellow) residues 311–319 are shown connecting to H8A and residues 370–375 to H8B. No continuous density is observed beyond residue K319 until residue I370, indicating that the rest of the H8 loop is structurally disordered and no additional density is observed bound to the concave surface of the C-terminal arch. A zoomed-in view of the ordered portions of the H8 loop is shown in Figure S5.