Figure 4.

NPS decreases anxiety-like behavior through a CRF₁-independent mechanism. A, B, Light/dark transfer behaviors in response to NPS after pretreatment with vehicle or antalarmin in C57BL/6J mice (N = 16) (A) and in CRF₁ WT and KO mice (N = 20) (B). The left panels show the time (in seconds) spent in the light compartment, and the right panels show the number of transitions the mice made between the dark and light compartments of the apparatus. C, Marbles buried after NPS in CRF₁ WT and KO mice. The data are represented as means ± SEM. Statistical significance was calculated by two-way between-subjects ANOVA. *Indicates overall significant effect of treatment (saline vs NPS).