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. Author manuscript; available in PMC: 2012 Sep 10.
Published in final edited form as: Acta Neuropathol. 2011 Jun 1;122(3):285–292. doi: 10.1007/s00401-011-0843-x

Figure 2. PSD-95 decreases in apical dendrites in hippocampus in 14 month old, but not in 3 month old, JNPL3 mice.

Figure 2

a. PSD-95 (green) and tau (red) immunoreactivity with TO-PRO 3 (blue) nuclear counterstain in hippocampus of wild type (WT), 3 month old (3M) and 14 month old (14M) JNPL3 mice. Boxes indicate the portion of CA1 region illustrated in b and c. Bar for all = 500 μm. b. High power images show PSD-95 and tau double-labeling. In WT, PSD-95 immunoreactivity labels apical dendrites and no tau positive neurons are present. In 3M JNPL3, PSD-95 immunoreactivity also labels apical dendrites, with a number of tau positive neurons present. In 14M JNPL3, PSD-95 immunoreactivity disappears from apical dendrites and appears in a number of neuronal soma (arrow), some of which localize with tau (arrowhead). Bar for all = 50 μm. c. Images of 100 μm × 100 μm areas show TO-PRO 3 stained nuclei (upper panel) and PSD-95 labeled apical dendrites (lower panel) that were analyzed in d and e respectively. Bar for all = 50 μm. d. Numbers of neuronal nuclei counted from CA1 pyramidal layer do not differ among the 3 groups: WT = 24.2±0.7, 3M = 23.6 ±0.3, 14M = 23.2±0.2 (p > 0.05, ANOVA). e. PSD-95 intensity measured from CA1 apical dendrites is significantly reduced in 14M by 57.7±0.9% and 57.4±5.1% compared to WT and 3M JNPL3 mice, respectively (*: p < 0.001, post hoc Tukey test).