Figure 1.

DsiRNA delivery has no silencing effect in well-differentiated PAE cultures. (a) Well-differentiated PAE cultures were transfected with HPRT or NC1 DsiRNA (250 nmol/l) using PEI, Transductin or TAT-PEI, or Accell siRNA at the indicated concentrations. The cells were harvested for RNA after 24 hours, and reverse transcribed into cDNA, which was quantified by quantitative PCR (qPCR). All mRNA levels were normalized to NC1-treated samples. Mean levels (±SD) were calculated from three biological replicates (in triplicate). (b–c) Confocal images (x–z stacks) of epithelia 2 hours after transfection with DIG-HPRT DsiRNA complexed with Transductin (b) or without any transfection reagent (c). Blue, labeled nuclei; green, DIG-labeled oligo.