FIG. 6.

Mutations in the template core can alter translocation. The mutation ter1-8T (shown in gray, marked with a dot) can lead to the synthesis of two types of repeat. In the first type, the telomere base pairs with TR1-5 (shown in the upper alignment) and incorporates the mutation as a single-base change (shown in gray in the upper sequence). In the second type (lower alignment), base pairing occurs within the template and leads to the synthesis of a 20-bp repeat. Sequences from two telomeres cloned from the ter1-8T mutant are shown. Wild-type repeats (identified by the italicized nucleotide at position 7 and an asterisk) are interspersed with the ter1-8T and TER1-7C(Bcl) repeats (which have a C at position 7) made by telomerase alleles in the cell.