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. 2012 Aug 20;109(36):14693–14698. doi: 10.1073/pnas.1212547109

Fig. 2.

Fig. 2.

ROP11/ARAC10 interactions with GEFs and ABI2 and expression patterns of ROP11/ARAC10, GEF1, and FER. (A) Y2H assays showing WT or CA-ROP11/ARAC10, but not DN-ROP11/ARAC10, interacting with ABI2. (B) DN-ROP11/ARAC10 interacts with full-length GEF1, GEF4, and GEF10 in the Y2H assays. (C) ROP11/ARAC10 and ABI2 interacted in BiFC assays in Arabidopsis protoplasts. (a) Interacting nVenus–ROP11/ARAC10 and ABI2–cCFP was observed as GFP image. (d) Negative control (nVenus–ROP11/ARAC10 with cCFP vector) was shown. (b and e) Chloroplasts are indicated by red auto-fluorescence. (c and f) Merged images of GFP and red signals are shown. (Scale bars: 10 μm.) (D a–d) The expression of ROP11/ARAC10–GUS reporter in leaves (a), guard cells (b), roots (c), and pollen (d). (e–g) GEF1–GUS expression was also detected in leaves (e), guard cells (f), and roots (g). (h) ProFER-FER-GFP was expressed in epidermal and guard cells. (Scale bars: b–d, f, and g, 20 μm; a and e, 0.5 cm).