Fig. 5.

The kinase domain of SID-3 is required for the efficient import of dsRNA into cells. sid-3(−) animals that express GFP in the pharynx and in bwm cells but that express gfp-dsRNA only in the pharynx (A) were transformed with constructs that express either wild-type SID-3 [sid-3(+)] (B) or a kinase-dead version of SID-3 [sid-3(KD−)] (C) in bwm cells. Representative fluorescence images of silencing in response to gfp feeding RNAi in third larval-staged animals of each of the above three genotypes are shown. Only animals that express sid-3(+) in bwm showed silencing of bwm cells (brackets). Insets are brightfield images. (Scale bars, 50 μm.)