Skip to main content
. 2012 Aug 20;109(36):14399–14404. doi: 10.1073/pnas.1210465109

Fig. 5.

Fig. 5.

AMP and hydrolysable analogs of cAMP inhibited TbTOR4 expression and induced the stumpy-like quiescent form. (A) The membrane-permeable and hydrolysable cAMP analog (8-pCPT-2′-O-Me-cAMP) (10 μM) down-regulates TbTOR4 expression and induces stumpy-like (pH5.5-resistant) cells in monomorphic trypanosomes; TbTOR1 expression is unaffected. Anti-tubulin was used as a loading control. (B) The hydrolysis-resistant cAMP analog (Sp-8-pCPT-2′-O-Me-cAMPS) (10 μM) does not affect TbTOR4 expression levels or induction of stumpy-like cells. Anti-tubulin was used as a loading control. (C) Membrane permeable 5′-AMP (8-pCPT-2′-O-Me-5′-AMP) (5 μM) promotes rapid TbTOR4 down-regulation and induces stumpy-like monomorphic trypanosomes. Anti-TbRRP4 was used as a loading control (31).