Fig. 4.

Hnf4a binding to the DNA regions of selected genes as measured by ChIP. Binding to promoters of Hnf4a, Chrebp, Cyp2e1, Cyp8b1, and Scd1 genes by Hnf4a was significantly lower in KO compared with WT livers. The genes examined were selected from genes down-regulated in the absence of adiponectin that had putative Hnf4a binding sites in their promoter regions. Insulin exon was used as negative controls. Data are presented as the mean ± SEM of four replicates from at least two independent ChIP experiments. *0.01 < P < 0.05; **0.001 < P < 0.01; ***P < 0.001.