Fig. 3.

Anti-FSH antibody (FSH Ab) inhibits bone resorption and stimulates bone synthesis. Tartrate-resistant acid phosphatase-labeled surfaces (pink) (A) and xylelol orange/calcein double-labeled surfaces (B) in bone sections (Materials and Methods) from mice treated with FSH Ab (100 μg/d) or IgG for 4 wk following sham operation (Sham) or ovariectomy (OVX). Representative double-labeled surfaces are magnified to show differences in interlabel distances. Bone resorption and formation parameters, namely resorption surfaces (resorbed S/BPm) (C), mineralizing surface (MS), mineral apposition rates (MAR), and bone formation rates (BFR) (D) are shown. Statistics by Student t test with Bonferroni’s correction; comparisons of FSH Ab-treated mice against IgG, *P ≤ 0.05, **P ≤ 0.01; other comparisons noted; ^P ≤ 0.05, ^^P ≤ 0.01; mean ± SEM is shown, n = 8 mice per group.