Fig. 2.

Differences in metabolic intermediates present in the hemolymph of (A) nondiapausing and diapausing pupae and (B) after diapause termination in response to an injection of 20E. Metabolic intermediates, including trehalose, glucose, pyruvate, and four substances in the TCA cycle, were compared: day 3 nondiapause pupae (ND), day 21 diapausing pupae (D), and day 21 diapausing pupae injected with 1 μg 20E. Relative ratio is the ratio of the peak area of metabolic intermediate to the peak area of the internal standard (sucrose). Hemolymphs from 10 pupae were mixed as one sample; bars represent the mean ± SD of four replicates. *P < 0.05; **P < 0.01 (determined by one-way ANOVA).