FIG. 5.

The 13 unique residues at the N terminus are necessary for ΔNp73β to suppress cell growth. (A and B) Levels of induced expression of HA-ΔNp73β and ΔNp73β(Δ2-13) in H1299 (A) or MCF-7 (B) stable cell lines are comparable. Western blot analysis was performed by using anti-p73 (Ab-3) monoclonal antibody. (C and D) ΔNp73β(Δ2-13) is incapable of suppressing cell growth in H1299 cells (C) and in MCF-7 cells (D). (E and F) ΔNp73β(Δ2-13) is incapable of inducing cell death in H-1299 cells (E) and in MCF-7 cells (F). (G) ΔNp73β(Δ2-13) is incapable of inducing cell cycle arrest and apoptosis in H1299 cells.