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. 2004 Jan;24(2):502–513. doi: 10.1128/MCB.24.2.502-513.2004

FIG. 10.

FIG. 10.

Expression of coregulators modulates responsiveness to 9-cis-RA. (A) CV-1 cell were transfected with the thymidine kinase (TK)-chloramphenicol acetyltransferase (CAT) construct containing the PRL TRE and expression vectors for TR (200 ng) and RXR (50 ng). Vectors (100 ng) for the coactivators ACTR, TIF-2, SRC-1, and DRIP205 or a noncoding vector (100 ng) was cotransfected with the reporter and the receptors, and CAT activity was determined after treatment with T3 (5 nM) and/or 9-cis-RA (1 μM) for 48 h. Data are expressed as the factors by which induction exceeded the value obtained for the corresponding control untreated cells. (B) Pituitary GH4C1 cells were cotransfected with the PRL promoter construct and 200 ng of expression vectors for SMRT or NcoR. Luciferase activity was determined after incubation with 5 nM T3 and/or 1 μM 9-cis-RA. In 235-1 cells the PRL reporter was cotransfected with 15 μg of TR and 500 ng of SMRT or NcoR.