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Expression of TR confers responsiveness to both T3 and 9-cis-RA in 235-1 cells. (A) The PRL construct was transfected into pituitary 235-1 cells together with 35 μg of an expression vector for TR or with the same amount of a noncoding vector. (B) The cells were cotransfected with the PRL promoter and either a wild-type receptor or the P363S mutant TR (20 μg). Reporter activity was measured after 48 h in the presence of 50 nM T3 and/or 1 μM 9-cis-RA, as indicated.
