FIG. 8.

The TR AF-2 domain is required for coactivator recruitment by the RXR ligand. (A) Gel retardation assays with the receptor-interacting domains of TIF-2 (top) and DRIP205 (bottom) fused to GST and the PRL TRE oligonucleotide. As indicated, wild-type (wt) TR and RXR, receptors lacking helix 12 (ΔAF2), and the point mutant TR TR(E401Q) were used. (B) Assays were performed with TR(E401Q), native RXR, and wild-type (Wt) His-tagged TIF-2 or TIF-2 with a mutation in the second LXXLL box (M2). (C) The consensus DR4 oligonucleotide was incubated with GST-TIF-2 and either wild-type receptors or TR(E401Q). In all panels experiments were performed in the presence and absence of T3 (20 nM) and 9-cis-RA (1 μM) as indicated.