FIG. 4.

Activated Akt inhibits tBID-induced BAX activation. (A) Rat1a, Rat1a-mAkt, and Rat1a-Bcl-2 cell lines were infected with control or tBID-expressing retrovirus for 12 or 16 h in the presence of 100 μM zVAD, and immunofluorescence using conformation-specific anti-BAX antibodies was performed following infection. Representative fields for all three cell lines are presented. (B) Quantitation of cells displaying BAX activation, as visualized in panel A. All data are depicted graphically as the means ± standard errors of the means for three independent experiments.