FIG. 3.

Adult wing phenotypes generated by PI3K_68D transgenes with Gal4-69B. (A) A control wing expressing the Gal4-69B promoter alone generates an essentially wild-type wing characterized by five longitudinal veins (L1 to L5), two cross veins (anterior [acv] and posterior [pcv]), and a number of sensory elements, including the campaniform sensillae (A and G) on the distal portion of L3 (L3-1 to L3-3). (B) Expression of WT-PI3K_68D transgenes produced a small hole (arrow) within the proximal part of the first basal cell in the hinge region. This unusual phenotype was completely penetrant (at 25°C) and was observed with six different insertions. Frequently a hole was also observed in the haltere (not shown). (C to F) Most flies expressing KD-PI3K_68D under control of the Gal4-69B promoter produced mild ectopic wing vein material (C), but in a proportion of flies (D to F) a more severe range of patterning defects was observed. In panel D, a single ectopic cross vein (arrow) is produced between the distal end of L2 and vein L3. In panel E, multiple ectopic cross-veins are formed and there is a thickening of the longitudinal veins in the anterior region. Panels E and F show losses of portions of the wing margin. In panel E, the sensory bristles associated with the anterior wing margin occur at a higher density in the region proximal to the wing margin loss. (G and H) Section of L3 distal to the anterior cross vein which carries the L3-1 to L3-3 campaniform sensillae (see panel A). Flies expressing KD-PI3K_68D show supernumary campaniform sensillae (arrows) (H) compared to controls (Gal4-69B only) (G). The wings shown are from female flies of genotypes Gal4-69B/+ (A and G), WT-PI3K_68D/+; Gal4-69B/+ (B), and Gal4-69B/UAS-KD-PI3K_68D (C to F and H). Anterior is up; proximal is to the left.