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. 2004 Jan;24(2):796–808. doi: 10.1128/MCB.24.2.796-808.2004

FIG. 5.

FIG. 5.

Genetic interactions between PI3K_68D deficiencies and components of the Drosophila EGFR and Notch signaling pathways. (Top) Region of chromosome arm containing the PI3K_68D gene. Deficiency mapping by Southern hybridization showed that the PI3K_68D locus is uncovered by the deficiencies Df(3L)vin3, Df(3L)vin4, Df(3L)vin6, and Df(3L)vin7 but excluded from Df(3L)vin2 and Df(3L)vin66, placing it at ∼68D6 to 68E3-4. This region probably contains five lethal complementation groups (31), including the genes cyclin A and brachyenteron. The chromosomal breakpoints of the vin deficiencies are from reference 1. (A to F) Notching of the wing margin by N264-39 (A) is enhanced by a deficiency, Df(3L)vin3, which deletes the class II PI3K, PI3K_68D (B), but not by Dp110A, which removes the class I PI3K, PI3K_92E (C). Similarly, the loss of the L4 wing vein on ectopic expression of argos (D) is rescued by Df(3L)vin3 (E) but unaffected by Dp110A (F). Crosses were performed at 25°C. The wings shown are from female flies of genotypes N264-39/+ (A), N264-39/+; Df(3L)vin3/+ (B), N264-39/+; Dp110A/+ (C), Gal4-69B, UAS-aos/+ (D), Gal4-69B, UAS-aos/Df(3L)vin3 (E), and Gal4-69B, UAS-aos/Dp110A (F).