Figure 4.

Real-time TaqMan RT-PCR analyses of EV71 RNA transcripts. RD cells were transfected with unmodified and 2′-modified siRNAs targeting the 5′ UTR of the EV71 genome and then infected with 0.01 MOI of EV71. At 36 h post-infection, viral RNA was extracted from the RD cells. The EV71 RNA transcript levels are shown relative to the mock transfection control in percentages. All data are presented as means ± SD from three independent experiments, each performed in duplicate. ^*P < 0.05, compared with mock transfection control. ^#P < 0.05, compared with 50 nM of si-1, si-1OMe, si-1 F, si-2, si-2OMe, and si-2 F, respectively.