Table 1.
Nucleotide sequences of siRNAs and their target positions in the EV71 genome (Genbank accession no. HM003207.1)
| siRNA | Chemically modified | Nucleotide sequence | Genomic position |
|---|---|---|---|
| si-1 |
No |
5′-CAGCAAACCACGAUCAAUATT-3′(Sense) |
115-133 |
| |
|
5′-UAUUGAUCGUGGUUUGCUGTT-3′(Anti-sense) |
|
| si-1OMe |
2′-OMe-modified |
5′-CAGCAAACCACGAUCAAUATT-3′(Sense) |
115-133 |
| |
|
5′-UAUUGAUCGUGGUUUGCUGTT-3′(Anti-sense) |
|
| si-1 F |
2′-F-modified |
5′-CAGCAAACCACGAUCAAUATT-3′(Sense) |
115-133 |
| |
|
5′-UAUUGAUCGUGGUUUGCUGTT-3′(Anti-sense) |
|
| si-2 |
No |
5′-CAGAGCAAUUGUUUACCUATT-3′(Sense) |
648-666 |
| |
|
5′-UAGGUAAACAAUUGCUCUGTT-3′(Anti-sense) |
|
| si -2OMe |
2′-OMe-modified |
5′-CAGAGCAAUUGUUUACCUATT-3′(Sense) |
648-666 |
| |
|
5′-UAGGUAAACAAUUGCUCUGTT-3′(Anti-sense) |
|
| si-2 F |
2′-F-modified |
5′-CAGAGCAAUUGUUUACCUATT-3′(Sense) |
648-666 |
| |
|
5′-UAGGUAAACAAUUGCUCUGTT-3′(Anti-sense) |
|
| scr |
No |
5′-GGAUUGACCUCUCAUAUAATT-3′(Sense) |
|
| |
|
5′-UUAUAUGAGAGGUCAAUCCTT-3′(Anti-sense) |
|
| si-2FAM |
FAM-modified |
5′-(FAM) CAGAGCAAUUGUUUACCUATT-3′(Sense) |
648-666 |
| 5′-UAGGUAAACAAUUGCUCUGTT-3′(Anti-sense) |
*Italicized letters indicate the positions of chemically modified nucleotides.