Figure 1.

Sequence composition of the conserved homeodomain in Phx1 and its subcellular localization.(A) Multiple sequence alignment of the homeodomain (HD; 167–227) of Phx1 with those of other fungi; Hoy1p of Yarrowia lipolytica (Yl), Pah1p of Podospora anserina (Pa), Pho2p of S. cerevisiae (Sc), Wariai of Dictyostelium discoideum (Dd). The sequences were aligned using Vector NTI AlignX program (Invitrogen Co.). The three α-helices are indicated above and the consensus was shown at the bottom. The sequences were retrieved from the GenBank database. [CAA93700, CAA84415, CAC16792, CAA64906, AAB92245 for Phx1, Hoy1p, Pah1p, Pho2p, Wariai respectively]. (B) Localization of Phx1-GFP. Cells containing the chromosomally integrated fusion gene for Phx1-GFP were grown in liquid EMM at 30°C. Aliquots taken during the exponential (OD₆₀₀ of 1, at around 18 h culture) and stationary (OD₆₀₀ of 8–9, at around 42 h culture) phases were examined for fluorescence and DIC images by fluorescence microscopy (Axiovert 200 M, Carl Zeiss).