FIG. 2.

Molecular characterization of Ctrn-deficient mice. (A) Northern blot analysis of RNA from various adult wild-type tissues (mRNA; left panels) as well as from the hearts, kidneys, and livers of 8- to 12-week-old Ctrn-deficient littermates (total RNA; right panels). Mouse cDNA probes for citrin (Slc25a13), aralar (Slc25a12), ASS (Ass1), PSTI (PSTI/Spink3), and β-actin were used. H, heart; B, brain; Sp, spleen; Lu, lung; Li, liver; Sk, skeletal muscle; K, kidney; T, testis. (B) Western blot analysis of liver, kidney, and heart protein extracts from 3-week-old Ctrn-deficient mice. Antibodies specific for citrin, ASS, and aralar were used.