Table 1.
Comparative sequence analysis of single GS/M trophozoites at thetpilocus
|
Isolate |
Material |
DNAreleasy |
GenBank acc no |
Nucleotide position from start of gene |
||
|---|---|---|---|---|---|---|
| 39* | 45 | 264 | ||||
|
GS/M |
Cloned sequence |
|
EF688030 |
A |
T |
G |
| |
Cloned sequence |
|
EF688028 |
G |
C |
A |
| |
Crude isolate |
|
FJ560571 |
R |
Y |
R |
|
GS/M |
Crude isolate |
|
N/A |
R |
Y |
R |
|
GS/M_3 |
Single trophozoites |
Not used |
N/A |
G |
C |
A |
|
GS/M_5 |
|
|
|
G |
C |
A |
|
GS/M_7 |
|
|
|
G |
C |
A |
|
GS/M_8 |
Single trophozoite |
Not used |
N/A |
A |
T |
G |
|
GS/M_6 |
Single trophozoite |
Not used |
N/A |
R |
Y |
R |
|
GS/M_71 |
Single trophozoites |
Used |
JN579671 |
R |
Y |
R |
|
GS/M_72 |
|
|
|
R |
Y |
R |
|
GS/M_73 |
|
|
|
R |
Y |
R |
|
GS/M_74 |
|
|
|
R |
Y |
R |
|
GS/M_76 |
|
|
|
R |
Y |
R |
|
GS/M_77 |
|
|
|
R |
Y |
R |
|
GS/M_78 |
|
|
|
R |
Y |
R |
|
GS/M_79 |
|
|
|
R |
Y |
R |
| GS/M_80 | R | Y | R | |||
* This nucleotide position is a substitution pattern proposed as a marker for different B sub-assemblages [25].