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. 2012 Jun 20;12:112. doi: 10.1186/1471-2180-12-112

Table 1.

qRT-PCR primers used in this study

Primer name Sequence Characteristics References
5RTRRSB
5’-TGCAAGTCGAACGGTAACAG-3’
qRT-PCR rrsb gene
[40]
3RTRRSB
5’-AGTTATCCCCCTCCATCAGG-3’
rpoS Fw
5’-AGTCAGAATACGCTGAAAGTTCATG-3’
qRT-PCR rpoS gene
[41]
rpoS Rv
5’-AAGGTAAAGCTGAGTCGCGTC-3’
iutAFw
5’- GATCATAGTGTCTGCCAGCC-3’
qRT-PCR iutA gene
This study
iutARv
5’- GCTCTTTACCGCCCTGAATC-3’
iutAO104_F
5’-ATGGAGTTTGAGGCTGGCAC-3’
iutA mutant confirmation
This study
iutAO104_R 5’-GCTTACTGTCGCTGACGTTC-3’