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. 2012 Jun 13;12:107. doi: 10.1186/1471-2180-12-107

Figure 1.

Figure 1

A) Inhibition of plasmodial DHS by RNAi and monitoring of the 612 bp amplificate by RT-PCR after transfection of 293 T cells with the DHS expression vector. 293T cells were cotransfected with: 1) Scramble II-duplex shRNA; 2) no transfected DNA; 3) the recombinant pcDNA3 vector containing 612 bp of a -highly conserved region of the dhs gene from P. falciparum (amino acid positions 208–412); 4) DHS- shRNA construct P#176; 5) DHS- shRNA construct P#43. B) Analysis of the 983 bp GAPDH amplificate in the cotransfected 293T cells described in Figure 1A.