Figure 2.

CYPs, MAO-A steady-state kinetics with PQ. Intitial velocity (Vo) vs. PQ concentration. Concentrations were as follows: PQ was from 0 to 40 mM, CYPs, MAOs were at 100 mg/ml, NADP was 1.3 mM, MgCl₂ and G6P were both 3.3 mM, and G6PD was 1 Unit/ml. Reaction mixture was incubated for 30 min and quenched with cold ACN. Each experiment was done with an n of four to eight. Error reported as SD.