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Identification of PhoA by mass spectrometry. A tryptic digest of the 2-D gel spot was analysed by MALDI-TOF to obtain a ‘peptide mass fingerprint’ that was subsequently searched against the NCBI database (Taxonomy = Bacteria). The only significant matches were to AP sequences. The sequence shown is PhoA, and the matched peptides are underlined. The predicted signal peptide is double underlined. The 16 matched peptides are shown in the table below.
