Figure 4 .

Median lifespan is reduced inM6mutant flies. (A) Schematic diagram (not drawn to scale) of the M6 locus showing the localization of independent P-element insertions (arrowheads). Start transcription sites corresponding to P1 and P2 and the stop codon (TAA) are also indicated. (B) Survival curves of adult w, M6⁰²/ ⁰², heterozygous and homozygous M6⁰¹ males (M6⁰¹/ ⁺ and M6⁰¹/ ⁰¹, respectively). The percentage of surviving flies ± SE over time (in days) at 25 °C in LD (standard conditions) is shown. Statistical analysis included a Log-rank (Mantel-Cox) survival curve comparison (p < 0.0001; Graphpad Software). Sample size ranged between 292 and 496 flies per genotype.