Table 1.
Summary of RT-PCR reactions performed for selected TDFsa
| TDF ID | Description | Category | EST | cDNA-AFLPb | Sizì (bp) | RT-PCRd |
|---|---|---|---|---|---|---|
| 02 C11 |
Hypothetical |
Unclassified |
DT468917 |
up R |
161 |
N |
| 02 H11 |
Hypothetical |
DW510601 |
up R |
208 |
Y |
|
| 02A12 |
Hypothetical |
DW498397 |
up R |
244 |
Y |
|
| 06E08 |
no homology |
na* |
up R |
186 |
Y |
|
| 06B01 |
glycosyl transferase family 1 protein |
ES813597 |
up L |
225 |
N |
|
| 01D09 |
nucleic acid binding protein/cold shock induced |
Stress/Defense |
ES820443 |
up R |
268 |
Y |
| 01B11 |
chloroplast heat shock protein 70.1 |
DT572113 |
up L up R |
176 |
Y |
|
| 02D03 |
glutathione S-transferase |
DW495591 |
up L |
320 |
N |
|
| 02A05 |
Lea5-D/Drought induced |
DW228770 |
up R |
233 |
Y |
|
| 02A09 |
manganese superoxide dismutase-like protein |
DW226492 |
dn R |
209 |
N |
|
| 03E11 |
universal stress protein |
DW484973 |
up R |
350 |
N |
|
| 04B09 |
Hsp70 |
DW486235 |
up R |
275 |
Y |
|
| 02 C06 |
phosphoethanolamine N-methyltransferase |
DW238205 |
up R |
505 |
N |
|
| 07B12 |
phosphoadenosine phosphosulfate reductase family protein |
EY197850 |
up R |
273 |
Y |
|
| A04H12 |
Cu/Zn SOD |
JG454758 |
up R |
338 |
Y |
|
| 03A07 |
beta-ketoacyl-ACP synthase II |
Metabolism |
DW238354 |
up R |
333 |
Y |
| 08 H05 |
ribose-5-phosphate isomerase |
DT556372 |
up L |
227 |
Y |
|
| 08D03 |
mannose-6-phosphate isomerase |
DW497531 |
up R |
175 |
N |
|
| 03 H01 |
MADS-box protein (AGL84) |
Gene Regulation |
ES839612 |
up L |
151 |
N |
| 02 F09 |
translation initiation factor-like protein |
EX172706 |
up R |
230 |
Y |
|
| 04 G11 |
RAN2; GTP binding |
Signal Transduction |
ES827152 |
dn R |
180 |
Y |
| 08 H03 |
Lipoxigenase 3 |
DT567650 |
up R |
190 |
Y |
|
| 03 F02 |
oligo Peptide transporter, putative |
Transport |
DW502711 |
dn R |
200 |
N |
| 06 C03 |
aquaporin PIP1;3/1;4 |
DQ402075 |
up R |
466 |
Y |
|
| 06E09 |
aquaporin PIP1;12 |
GU998829 |
up L |
313 |
N |
|
| |
|
|
dn R |
|
|
|
| 08A01 |
NITRATE TRANSPORTER1/PEPTIDE TRANSPORTER (POT/NAXT) family |
na |
dn R |
300 |
Y |
|
| 04D09 |
Serine carboxypeptidase |
Protein Fate |
DT456670 |
dn R |
200 |
Y |
| 01E10 |
Photosystem I reaction center subunit VI |
Photosynthesis |
EY197220 |
up L |
189 |
N |
| 07E12 |
Cytochrome b6-f complex |
|
ES845215 |
dn L |
285 |
N |
| 07E02 |
hydrolase |
Cell Growth/Structure |
DT465622 |
dn L |
189 |
N |
| A5B01 | Expansin | DW494258 | dn L | 278 | Y |
aAll reactions were replicated at least twice and two separate gel electrophoresis were performed per reaction.
bup and dn represent up-regulation and down-regulation, respectively in root (R) and leaf (L) tissues.
cexpected size from PCR amplification.
dRT-PCR results which correspond to cDNA-AFLP data (Y) and do not (N).
*Not available.