TABLE 2.
Influence of inactivation of Hsp70.1/3 on chromosome aberrationsa
| Mouse genotype | Heat | Chromosome gaps + breaks | Chromatid gaps + breaks |
|---|---|---|---|
| Hsp70.1/3+/+ | − | 2 | 3 |
| Hsp70.1/3+/+ | + | 3 | 5 |
| Hsp70.1/3−/− | − | 7* | 9* |
| Hsp70.1/3−/− | + | 10* | 23* |
a
Age-matched Hsp70.1/3−/− and Hsp70.1/3+/+ mice (four sets of both sexes for each genotype) were subjected to heat treatment for 30 min at 41°C and then kept at room temperature for 24 h. Colcemid was administered 4 h prior to collection of bone marrow. Metaphases were made by standard procedures. Two hundred metaphases were examined for each mouse type, and the number of breaks per 100 metaphases is reported for each case. Frequencies for chromosomal or chromatid types of aberrations in Hsp70.1/3−/− mouse cells are significantly higher than those in Hsp70.1/3+/+ control mice as assessed by chi-square analysis (P < 0.05) (marked by asterisks).