Table 1. List of primer pairs and PCR annealing temperatures used in the present study for the three selected DNA barcoding markers.
Locus | Code | Primer name | Sequences (5′-3′) | Annealing temperature | Reference |
rbcL | - | 1F | ATGTCACCACAAACAGAAAC | 50°C | [42] |
724R | TCGCATGTACCTGCAGTAGC | [42] | |||
matK * | A | 390F | CGATCTATTCATTCAATATTTC | 53°C | [43] |
1326R | TCTAGCACACGAAAGTCGAAGT | [43] | |||
B | XF | AATTTACGATCAATTCATTC | 50°C | [44] | |
5R | GTTCTAGCACAAGAAAGTCG | [44] | |||
C | 1R_KIM | CGTACAGTACTTTTGTGTTTACGAG | 55°C | Ki-Joong Kim, unpublished | |
3F_KIM | ACCCAGTCCATCTGGAAATCTTGGTTC | Ki-Joong Kim, unpublished | |||
trnH-psbA | - | psbA | GTTATGCATGAACGTAATGCTC | 53°C | [24] |
trnH | CGCGCATGGTGGATTCACAAATC | [24] |
each pair of primers was used according to [36].